Szleifer: Thermodynamcis and Kinetics of Protein Adsorption
Nov
3
Mon 4:00 PM
When Monday, November 3, 2008
Time
4:00 PM - 5:00 PM
Where Tech M416
Contact Molly Scanlon
847-491-5586
Group McCormick-Colloquia Engineering Sciences and Applied Mathematics
Applied Math Colloquium
Title: Thermodynamcis and Kinetics of Protein Adsorption
Speaker: Professor Igal Szleifer, Northwestern University
Abstract: Non-specific protein adsorption is the first process in the foreign body response. Biomaterials need to provide non-fouling surfaces to prevent adsorption of blood proteins. The molecular design of surface modifiers that prevent non-specific adsorption requires the understanding of the factors that determine protein adsorption. The hierarchy of time and length scales present in the adsorption requires a multiscale approach to treat the complexity of the process. We will discuss the driving forces that determine protein adsorption and how end-grafted polymers can be used to modify the ability of the proteins to reach the surface. We will show the differences between preventing protein adsorption thermodynamically and kinetically. In particular, we will demonstrate that polymer molecular weight plays no role for the thermodynamic control while it is very important in determining the kinetics of protein adsorption. We will discuss the relevance of short time scales to understand how proteins interact with surfaces. To this end we use atomistic molecular dynamics that enable the study of a single protein in the presence of a surface and explicit water over time scales of tens of nanoseconds. For practical applications the relevant time scales are hours or days. We will show how a coarse grained molecular approach can be used to study these time scales covering 16 orders of magnitude in time. Comparisons with experimental observations will be presented for the cases that are available.
Title: Thermodynamcis and Kinetics of Protein Adsorption
Speaker: Professor Igal Szleifer, Northwestern University
Abstract: Non-specific protein adsorption is the first process in the foreign body response. Biomaterials need to provide non-fouling surfaces to prevent adsorption of blood proteins. The molecular design of surface modifiers that prevent non-specific adsorption requires the understanding of the factors that determine protein adsorption. The hierarchy of time and length scales present in the adsorption requires a multiscale approach to treat the complexity of the process. We will discuss the driving forces that determine protein adsorption and how end-grafted polymers can be used to modify the ability of the proteins to reach the surface. We will show the differences between preventing protein adsorption thermodynamically and kinetically. In particular, we will demonstrate that polymer molecular weight plays no role for the thermodynamic control while it is very important in determining the kinetics of protein adsorption. We will discuss the relevance of short time scales to understand how proteins interact with surfaces. To this end we use atomistic molecular dynamics that enable the study of a single protein in the presence of a surface and explicit water over time scales of tens of nanoseconds. For practical applications the relevant time scales are hours or days. We will show how a coarse grained molecular approach can be used to study these time scales covering 16 orders of magnitude in time. Comparisons with experimental observations will be presented for the cases that are available.
