Description:

Guest speaker Matthew Schipma of the Next Generation Sequencing Core will lead a discussion on ChIP-seq techniques and best practices.

Gene expression is a highly regulated process involving several protein-DNA interactions. Transcription factors initiate selective gene expression by binding to specific DNA loci and recruiting other factors and RNA polymerase II. In addition, histones may wrap specific regions of DNA, thereby preventing transcription factors from binding. ChIP-seq provides a way to identify genome-wide binding sites of a particular protein. Here we will discuss the information that is can be gained through ChIP-seq as well as experimental considerations.