When:
Thursday, September 8, 2016
3:00 PM - 4:00 PM CT
Where: Robert H Lurie Medical Research Center, Searle Seminar Room, 303 E. Superior, Chicago, IL 60611 map it
Audience: Faculty/Staff - Student - Post Docs/Docs - Graduate Students
Contact:
Carson Nestler
(312) 503-5229
Group: Biochemistry & Molecular Genetics Seminar Series
Category: Lectures & Meetings
The Department of Biochemistry and Molecular Genetics Departmental Seminar Series presents:
Raymond Cho, MD, PhD
Associate Professor
Department of Dermatology
University of California San Francisco School of Medicine
Using a novel, high-content screening system in human keratinocytes, we have identified more than 60 transcription factors whose absence causes morphological abnormalities during calcium-induced differentiation. Importantly, we detected genes known to be critical to central keratinocyte functions, those underlying rare, new ectodermal diseases, and many with diverse, undescribed phenotypes. We have further validated 12 transcription factors whose silencing profoundly enhances the cell size and angulation characteristic of calcium-induced differentiation. We further established that this specific, abnormal cytoarchitectural phenotype also strikingly predicts activation of known keratinocyte development genes, including key subsets of the epidermal differentiation complex. We will discuss the implications of this screen more broadly, and also our specific results, on understanding and treating epithelial disease.