Description:

Illumina Next-Generation Sequencing Seminar
Sponsored by the NUSeq Core

There will be two separate sessions and researchers are invited to attend one or both sessions.

2:00 PM - Session I
Introduction to sequencing based methods utilizing the HiSeq 4000 and MiSeq sequencers from Illumina

Next-gen sequencing (NGS) is a major empowering technology for life science research and genomic medicine. NUSeq offers full NGS support for all Northwestern projects, from sequencing library construction, in-house Illumina sequencing, and bioinformatics data analysis. With the recent acquisition of Illumina HiSeq 4000 and MiSeq, NUSeq has a fleet of sequencers that meet every project's needs. In this seminar we will explore the use of the HiSeq 4000 for whole genome and exome sequencing. We will also discuss metagenomics applications on the MiSeq.

3:00 PM - Session II
Best Practices for Illumina Library Preparations

An Illumina Field Applications Scientist will discuss what goes into generating a successful library for sequencing on all Illumina platforms. The discussion will include quality control of DNA/RNA input, detection and removal of adapter dimer, the importance of utilizing dual index strategies, and index pooling guidelines. The differences between the Illumina sequencing platforms will be highlighted to help identify what sequencer is appropriate for specific projects.

Speakers Include:

Alasdair Reid, Ph.D., Sr. Sales Specialist, Sequencing and Data Analysis, Illumina, Inc.

Katelyn Michelini, Field Applications Scientist, Illumina, Inc.