When:
Tuesday, September 18, 2018
12:00 PM - 1:00 PM CT
Where: Robert H Lurie Medical Research Center, Baldwin Auditorium, 303 E. Superior, Chicago, IL 60611 map it
Audience: Faculty/Staff - Student - Post Docs/Docs - Graduate Students
Contact:
Dr. Derek Walsh
(312) 503-4292
Group: Department of Microbiology-Immunology Seminars/Events
Category: Lectures & Meetings
Title: "RNA Virus Evasion of Nonsense-Mediated Decay"
Anne Simon, PhD - University of Maryland
Host Derek Walsh PhD
Description:
Nonsense-mediated decay (NMD) serves a critical role in preserving the integrity of the host transcriptome. RNA transcripts bearing premature termination codons (PTCs) are subject to NMD as they can lead to expression of truncated, deleterious proteins, which is associated with many genetic diseases and cancer. While it is known that long 3’ UTRs promote NMD, many transcripts that naturally contain long 3’ UTRs are protected, but the mechanism(s) involved in this protection are virtually unknown. Natural stop codons in the genomes of multicistronic RNA plant viruses would resemble PTCs and thus these viruses must have developed the means to combat NMD to maintain genome stability. We show that the compact, positive-sense Turnip crinkle virus evades NMD by several mechanisms including having an unstructured region of RNA immediately downstream of the coat protein (CP) termination codon, which may also be used by non-susceptible host mRNAs.