When:
Monday, April 1, 2019
4:00 PM - 5:00 PM CT
Where: Pancoe-NSUHS Life Sciences Pavilion, Auditorium, 2200 Campus Drive, Evanston, IL 60208 map it
Audience: Faculty/Staff - Student - Post Docs/Docs - Graduate Students
Cost: Free
Contact:
Penelope Johnson
(847) 467-7464
Group: Chemistry of Life Processes Institute
Category: Academic
Chemical Biology on the Brain: Understanding the Nicotine Receptor
While structural studies continue to advance, obtaining detailed insights into small molecule binding interactions to complex, integral membrane receptors of the mammalian central nervous system remain a significant challenge. Using unnatural amino acid mutagenesis and heterologous expression in Xenopus oocytes, we have developed ways to address specific questions concerning drug-receptor interactions and other features of the nicotinic acetylcholine receptors (nAChR), a prototype receptor system. We can semi-quantitatively evaluate cation-π interactions, hydrophobic interactions, and hydrogen bonding interactions, both to amino acid side chains and to the protein backbone. This provides a general strategy for understanding drug-receptor interactions with chemical precision.