Description:

Special Seminar co-sponsored by CDB and Center for Advanced Microscopy:

"Scanned Oblique Plane Illumination Microscopy"

Dr. Genia Kozorovotskiy, Assistant Professor Department of Neurobiology, Northwestern University, Evanston

and 

Dr. Manish Kumar, Postdoctoral Researcher, Department of Neurobiology, Northwestern University, Evanston

2:00 - 3:00 PM

Wednesday, February 27, 2020

Lurie 1-123, Baldwin Auditorium

Abstract

Light-sheet microscopy has rapidly become a widely used imaging technique for studying biological samples, ranging from tissue culture, to organoids, to entire organisms. One relatively recent advance in the field, where our laboratory has made substantial contributions, involves the development of single front facing objective based configurations. This approach solves the problem of steric access associated with conventional light-sheet techniques, enabling imaging larger and more varied samples and organisms. Light-sheet microscopy is especially useful for experiments where point scanning techniques are too slow, where 3D information is essential, or where broad illumination induces excessive photobleaching. 

We will review the history and principles of light-sheet imaging, describing the key variants of oblique light-sheet microscopy, including our contribution, the scanned oblique plane illumination (SOPi) microscopy platform, which attains rapid (10+ VPS) imaging of fluorescently labeled structures and genetically encoded sensors at a relatively low cost with flexible design options. A detailed review of system geometry will highlight distinctions between currently published models, as well as both the strengths and the weaknesses of this class of techniques. We will review several open source tools for design, control, and optical alignment of new systems that we are introducing to facilitate broad adoption of SOPi-like imaging modalities at NU and beyond, as well as discuss current applications in neuroscience and biology, along with future development plans. 

References:

1.     M. Kumar, S. Kishore, J. Nasenbeny, D.L. McLean, and Y. Kozorovitskiy, "Integrated one- and two-photon scanned oblique plane illumination (SOPi) microscopy for rapid volumetric imaging," Opt. Express 26, 13027-13041 (2018) 

2.     M. Kumar and Y. Kozorovitskiy, "Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging," Opt. Lett. 44, 1706-1709 (2019)

3.     M. Kumar and Y. Kozorovitskiy, “Crossbill Design,” Zenodo (2019) https://doi.org/10.5281/zenodo.3543786

4.     M. Kumar and Y. Kozorovitskiy, Geometry of tilt (in)variance in scanned oblique plane microscopy. arXiv: 2001.00246