Description:

Title:

“Probing Birth and Death of mRNAs in Bacteria Using Single-Molecule Microscopy” 

Speaker: Sangjin Kim, PhD / University of Illinois Urbana-Champaign

Host: Hank Seifert, PhD

Topic:

My lab studies how transcription, translation, and mRNA degradation processes are coordinated within bacterial cells. I will provide a general overview of the single-molecule experiments we perform to probe gene expression in bacteria and dive into a story about how RNA polymerases work together during transcription elongation in E. coli. While a variety of molecular players are known to control transcription elongation, whether RNAPs themselves can regulate each other’s motion on the chromosome remains unclear. I will show in vitro and in vivo evidence suggesting that co-transcribing RNAPs can affect each other’s dynamics, either positively or negatively, through transcription-induced DNA supercoiling. 

Zoom:

https://northwestern.zoom.us/j/96130387201?pwd=OVQrYXhtV1dOdFNnTmVSVENmYUJXUT09

Meeting ID: 
961 3038 7201
Passcode:
641397