Northwestern Events Calendar

Sep
30
2022

Imaging Seminar by Dr. Irene Georgakoudi, Tufts University

When: Friday, September 30, 2022
11:00 AM - 12:15 PM CT

Where: Ryan Hall, 4003, 2190 Campus Drive, Evanston, IL 60208 map it

Audience: Faculty/Staff - Student - Public - Post Docs/Docs - Graduate Students

Cost: Free!

Contact: Benjamin D Keane   (847) 467-1870

Group: Center for Physical Genomics and Engineering (CPGE)

Category: Academic

Description:

Presented by the Center for Physical Genomics and Engineering, Robert H. Lurie Comprehensive Cancer Center, and McCormick Engineering.

Cellular metabolism is essential for cellular and tissue function. Thus, it is not surprising that numerous diseases are associated with metabolic dysfunction. Metabolic responses are highly complex and dynamic in space and time over a wide range of scales. It is challenging to capture this important aspect of metabolic function without introducing artifacts using traditional radiographic, exogenous label, or mass-spectroscopy-based imaging approaches. Label-free, non-linear optical imaging modalities offer unique opportunities to overcome at least partially some of these challenges. Two-photon excited fluorescence is particularly well-suited to assess cellular redox state and mitochondrial dynamics through the intensity and lifetime signatures of NAD(P)H and FAD, two key metabolic co-enzymes. While their participation in a number of metabolic pathways confers great sensitivity to changes in metabolic function, interpretation of the origins of optical signal changes lacks specificity. Potential contributions from other fluorophores further complicate the interpretation and robustness of these signals. Multi-parametric analysis of the optical images and/or combination with other modalities that provide more specific genomic or proteomic information provide interesting opportunities to improve our understanding of metabolic function and its role in health and disease. We have performed studies performed with a wide range of samples, including isolated mitochondria, engineering brain tissue models, excised human tissues and living humans. Our findings highlight the potential of label-free two-photon excited imaging to provide insights that may be useful for the improved diagnosis and treatment of conditions such as cancer, vitiligo, and traumatic brain injury.  Seminar will be in person and on Zoom at
https://northwestern.zoom.us/j/99453394437

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