Description:

Read alignment or mapping is a computational process to determine where in the reference genome the short RNA reads originated from. This workshop will introduce how to perform read alignment using STAR (Spliced Transcripts Alignment to a Reference), a splice-aware aligner designed to specifically tackle many challenges involved in RNA-seq data mapping. We will introduce STAR’s alignment strategy and interactively work through each step of alignment using an example dataset. We will also discuss how STAR is incorporated in many Nextflow pipelines (for example nf-core/rnaseq).

Prerequisites: This workshop assumes no prior knowledge related to RNA-seq data processing but requires a laptop with a terminal application installed , and expects familiarity with working from the command line or attendance at the ‘Command Line Introduction’ workshop.