Description:

Peak calling is a computational method used to identify areas in the genome that have been enriched with aligned reads in ChIPseq and ATACseq datasets. This workshop will introduce Model-based Analysis of ChIP-Seq (MACS2), one of the commonly used peak callers, and demonstrate how to call peaks from aligned reads on Quest. We will introduce some basic MACS2 parameters and interactively work through each step of peak calling using an example dataset.

Prerequisites: This workshop requires a laptop with a terminal application installed and basic knowledge of working from the command line, or attendance at the ‘Command Line Introduction’ workshop.