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DTSTART;TZID=America/Chicago:20251111T121500
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DTSTAMP:20261010T024705Z
SUMMARY:Microbiology-Immunology Seminar Series: Bercin Cenik\, PhD
UID:635254@northwestern.edu
TZID:America/Chicago
DESCRIPTION:Title: TurboCas: A method for locus-specific labeling of genomic regions and isolating their associated protein interactome  Description: The regulation of gene expression is a complex process that requires the concerted action of transcription factors and chromatin binding proteins. Because this process is both unique to a given locus and varied in response to changing cellular conditions\, dynamically mapping the chromatin binding activity at individual promoters and other regulatory loci is crucial to understanding how cis-regulatory elements control gene expression. Earlier methods could only characterize the static binding activity of a single given protein. However\, the recent emergence of proximity labeling\, a technique for interrogating protein-protein interactions\, and the advances brought about by CRISPR technology have enabled the development of new methods that can dynamically map the chromatin binding and secondary association of multiple proteins at given loci. In this study we describe TurboCas\, a method that leverages the latest generation of proximity labeling enzymes\, miniTurbo\, in combination with catalytically dead Cas9 to label chromatin-binding proteins efficiently\, dynamically and in a site-specific manner. We demonstrate the use of TurboCas to identify proteins binding the promoter of the heat shock responsive FOS gene. We go on to cross-validate our hits through an independent platform using RNA polymerase II and Cyclin T1 immunoprecipitation. Using these two methodologies\, we identify both canonical regulators of heat shock response and members of previously uncharacterized novel pathways as general heat shock regulators. TurboCas represents a significant improvement over previous locus-targeted proximity labelling methods\, with the potential not only to deepen our understanding of regulatory pathways in cellular stress response\, but more broadly to advance the transcriptional regulation and chromatin biology fields.
LOCATION:Robert H Lurie Medical Research Center\, Searle Seminar Room\, 303 E. Superior\, Chicago\, IL 60611
TRANSP:OPAQUE
URL:https://planitpurple.northwestern.edu/event/635254
CREATED:20251024T050000Z
STATUS:CONFIRMED
LAST-MODIFIED:20251024T050000Z
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