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DTSTART;TZID=America/Chicago:20260826T163000
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SUMMARY:"Molecule-scale resolution and dynamics in fluorescence microscopy" Stefan Hell\, PhD\, Max Planck Institute
UID:642999@northwestern.edu
TZID:America/Chicago
DESCRIPTION:CDB SPECIAL SEMINAR:  "Molecule-scale resolution and dynamics in fluorescence microscopy"  Wednesday\, August 26\, 2026  4:30 p.m. to 5:30 p.m.   Location to be announced     Stefan Hell\, PhD Director\, Max Planck Institute for Multidisciplinary Sciences in Göttingen\, and Max Planck Institute for Medical Research in Heidelberg\, Germany   I will discuss MINFLUX [1\, 2\, 3\, 4]\, a recent molecular localization and superresolution method that has reached Angström localization precision and resolution of the size of a fluorophore molecule. MINFLUX and the related MINSTED concept [5\, 6] are being established for routine applications in cell and molecular biology\, structural biology and neuroscience. Relying on much fewer fluorescence photons than the widely used camera-based localization methods\, these techniques are poised to characterize dynamic processes of single proteins\, as demonstrated by tracking the nanometer conformational changes of the motor proteins kinesin-1 [7] and dynein in living cells [8]. MINFLUX has also been demonstrated to measure intramolecular distances with Angström precision\, providing a precise and reliable alternative to FRET [9]. Harnessing confocal detection\, MINFLUX also provides nanometer-range resolution deeper down in layers of cells and (mildly) scattering tissue [10]. Finally\, I will show an arguably surprising ability of MINFLUX to separate individual identical fluorophores without sequential ON/OFF switching or activation of fluorescence. Thus\, the simultaneous\, uninterrupted\, nanometer-scale tracking and imaging of multiple\, identical (same-color) fluorophores becomes possible for the first time [11]. This novel superresolution principle should allow MINFLUX to reveal the conformational changes of individual proteins in their native environment.   [1] Balzarotti\, F.\, Eilers\, Y.\, Gwosch\, K. C.\, Gynnå\, A. H.\, Westphal\, V.\, Stefani\, F. D.\, Elf\, J.\, Hell\, S.W. Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes. Science 355\, 606-612 (2017).  [2] Eilers\, Y.\, Ta\, H.\, Gwosch\, K. C.\, Balzarotti\, F.\, Hell\, S. W. MINFLUX monitors rapid molecular jumps with superior spatiotemporal resolution. PNAS 115\, 6117-6122 (2018).  [3] Gwosch\, K. C.\, Pape\, J. K.\, Balzarotti\, F.\, Hoess\, P.\, Ellenberg\, J.\, Ries\, J.\, Hell\, S. W. MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells. Nat. Methods 17\, 217–224 (2020).  [4] Schmidt\, R.\, Weihs\, T.\, Wurm\, C. A.\, Jansen\, I.\, Rehman\, J.\, Sahl\, S. J.\, Hell\, S. W. (2021) MINFLUX nanometer-scale 3D imaging and microsecond-range tracking on a common fluorescence microscope. Nat. Commun. 12:1478.  [5] Weber\, M.\, Leutenegger\, M.\, Stoldt\, S.\, Jakobs\, S.\, Mihaila\, T. S.\, Butkevich\, A. N.\, Hell\, S. W. MINSTED fluorescence localization and nanoscopy. Nat. Photon. 15\, 361-366 (2021).  [6] Weber\, M.\, von der Emde\, H.\, Leutenegger\, M.\, Gunkel\, P.\, Sambandan\, S.\, Khan\, T. A.\, Keller-Findeisen\, J.\, Cordes\, V. C.\, Hell\, S.W. MINSTED nanoscopy enters the Ångström localization range. Nat. Biotechnol.\, 41\, 569-576 (2023).  [7] Wolff\, J. O.\, Scheiderer\, L.\, Engelhardt\, T.\, Engelhardt\, J.\, Matthias\, J.\, Hell\, S.W. MINFLUX dissects the unimpeded walking of kinesin-1. Science\, 379\, 1004-1010 (2023).  [8] Schleske\, J. M.\, Hubrich\, J.\, Wirth\, J. O.\, D’Este\, E.\, Engelhardt\, J.\, Hell\, S. W. MINFLUX reveals dynein stepping in live neurons. PNAS 121\, e2412241121 (2024).  [9] Sahl\, S. J.\, Matthias\, J.\, Inamdar\, K.\, Weber\, M.\, Khan\, T. A.\, Brüser\, C.\, Jakobs\, S.\, Becker\, S.\, Griesinger\, C.\, Broichhagen\, J.\, Hell\, S. W. Direct optical measurement of intramolecular distances with angstrom precision. Science 386\, 180-187 (2024).  [10] Moosmayer\, T.\, Kiszka\, K. A.\, Pape\, J. K.\, Leutenegger\, M.\, Steffens\, H.\, Grant\, S. G. N.\, Sahl\, S. J.\, Hell\, S. W. MINFLUX fluorescence nanoscopy in biological tissue. PNAS 121\, e2422020121 (2024).  [11] Hensel\, T. A.\, Wirth\, J. O.\, Schwarz\, O. L. Hell\, S. W. Diffraction minima resolve point scatterers at few hundredths of the wavelength. Nat. Phys. https://doi.org/10.1038/s41567-024-02760-1 (2025).
LOCATION:
TRANSP:OPAQUE
URL:https://planitpurple.northwestern.edu/event/642999
CREATED:20260626T050000Z
STATUS:CONFIRMED
LAST-MODIFIED:20260626T180501Z
PRIORITY:0
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