Description:

CRISPR/Cas9 is a new genomic editing system that has the potential to revolutionize biomedical research with its simple and efficient design. In this seminar, we will thoroughly examine the CRISPR system and some of its applications in the laboratory.

Topics will Include:

• CRISPR Design and Function
• Efficiency and Specificity
• Paired Nickases for Improved Specificity
• Gene Knockout, Insertion, and Modification
• RNA Format for Embryo Microinjection

Chris Lemke joined Sigma-Aldrich in 2009, and is a scientific liaison for translational research. He has lectured extensively on gene editing technologies, and is a subject matter expert on CRISPR/Cas9 and ZFN systems. Chris possesses a high level of expertise on topics of nuclease design, targeted gene knock-out and insertion, and principles of custom cell line and animal model development.