Northwestern Events Calendar

Oct
17
2022

Department of Pharmacology Seminar: Time-resolved Analysis of Gene Regulation in Single Cells, Hao Wu, PhD

When: Monday, October 17, 2022
3:00 PM - 4:00 PM Central

Where: Ward Building, Room 5-230, 303 E. Chicago Avenue, Chicago, IL 60611 map it

Audience: Faculty/Staff - Student - Public - Post Docs/Docs - Graduate Students

Contact: Melissa Daley  

Group: Department of Pharmacology Seminars

Category: Lectures & Meetings

Description:

Time-resolved Analysis of Gene Regulation in Single Cells

Gene expression programs change over time, development and maturation, and in response to stimuli. While gene regulation can be studied indirectly by quantifying total RNAs, directly measuring nascent or newly transcribed RNAs is required to reveal the immediate regulatory changes in response to developmental, environmental, and pathological signals. To gain a more complete mechanistic understanding of cell-type specific regulation of RNA dynamics in diverse biological processes, we have recently developed new technologies that can quantitatively distinguish newly synthesized (“new”) from pre-existing (“old”) RNAs in the same single cell at scale. We demonstrate that time-resolved single cell transcriptomics method can generate high temporal resolution RNA expression data (at the time scale of minutes to hours), which bring deeper insights into the molecular and cellular mechanisms in dynamic systems. Finally, by enabling quantitative analysis of mRNA kinetic parameters (synthesis, splicing & degradation), our approach can better define mechanistic link between RNA dynamics and cell state transition or any perturbation, thereby exploring the regulatory wiring of a cell beyond transcriptional control alone.

Hao Wu, PhD
Assistant Professor, Department of Genetics
Penn Epigenetics Institute
Penn Institute of Regenerative Medicine
University of Pennsylvania School of Medicine


Wu Lab WebsiteGoogle ScholarPenn Epigenetics Institute

 

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