Northwestern Events Calendar


M-I Dept. Seminar / Genome Instability Caused by Persistent RNA-DNA Hybrid Formation

When: Tuesday, December 5, 2023
10:00 AM - 11:00 AM CT

Where: Robert H Lurie Medical Research Center, Baldwin Auditorium, 303 E. Superior, Chicago, IL 60611 map it

Audience: Faculty/Staff - Post Docs/Docs - Graduate Students

Contact: Cynthia Naugles   (312) 503-0489

Group: Department of Microbiology-Immunology Seminars/Events

Category: Lectures & Meetings



Genome Instability Caused by Persistent RNA-DNA Hybrid Formation


Lyle SImmons, PhD, Professor, University of Michigan, Department of Molecular, Cellular and Developmental Biology


RNA-DNA hybrids form through DNA polymerase errors, Okazaki fragment primers, and as R-loops during transcription. Our lab has studied the effect of persistent ribonucleotides on genome integrity, using a combination of mutation accumulation lines, genetics, and in vitro reconstitution experiments. We have found that persistent ribonucleotide insertion leads to increased mutagenesis through an error-prone resynthesis pathway. Our work also shows that elevated R-loops cause gross chromosomal rearrangements and perturbations to DNA replication. Failure to properly resolve RNA-DNA hybrids results in slower growth and the acquisition of mutations that can lead to antibiotic resistance.


Hank Seifert, PhD, Professor, Dept. of Microbiology-Immunology

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